Contributions to Zoology, 71 (1/3) (2002)Peter Damen; Wim J.A.G. Dictus: Newly-discovered muscle in the larva of Patella coerulea (Mollusca, Gastropoda) suggests the presence of a larval extensor
Materials and methods

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Selection and fixation

Abnormal larvae at 48 to 52 h after first cleavage, i.e., larvae that do not possess eyes, a prototroch, a shell, a foot and an operculum or that are not able to retract into their shell, were discarded. The muscles of normal larvae of 48 to 52 h after first cleavage were relaxed by adding drops of 0.75 M MgCl2 to the MPFSW. Subsequently, the larvae were fixed in 4% formaldehyde in 0.1 M phosphate buffer (pH 7.4) for 1 h and rinsed in buffer (3 times for 10 min).