Material and methods
Nine adult females of Bufo ictericus Spix, 1824 were collected in accordance with Brazilian law (collecting permit no. 020222.003111/96-14 issued by IBAMA) in Rio de Janeiro State. The animals were killed with ether vapor after anesthesia . Ovarian and Bidder’s organ fragments were fixed by immersion in Bouin’s liquid for 18h at 4oC. After rinsing in ethanol 70%, the fragments were processed according to the standard histologic technique for paraffin embedding. Five micrometer serial slices were stained with hematoxylin-eosin and Gomori’s trichrome (Lillie and Fulmer, 1976).