Contributions to Zoology, 73 (4) (2004)C.F. Farias; L. de Brito-Gitirana*; S.P. Carvalho-e-Silva: Short notes and reviews

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Results

Macroscopically, the Bidder’s organ is observed in close association with the ovary (Figs. 2 and 3). The Bidder’s organs were found in seven females. In two females with fully grown postvitellogenic ovarian follicles, the Bidder’s organs could not be seen.

FIG2

Fig. 1–4. – 1– Photograph of a female of Bufo ictericus; 2– Macrograph of an immature female of Bufo ictericus. Note the close association of the ovary to the Bidder’s organ; 3 – Macrograph of a mature female of Bufo ictericus, but the ovarian follicles are not yet totally developed; 4 – Female of Bufo ictericus. Light micrograph showing close spatial relationship between the Bidder’s organ and the ovary. HE-staining.

The ovary consists of a thin sheath of connective tissue, enclosing the ovarian follicles and visualized at different stages of development (Fig. 4). The ovarian follicles consist of germ cells surrounded by a follicular cell layer. The germ cells display a large nucleus with several prominent nucleoli. The ovary is a sac-shaped structure without a typical medullar region.

Bidderian follicles at different stages of development are shown (Fig. 4). Germ cells display a rounded nucleus, and are enclosed by a layer of follicular cells, varying from flat to cubic. Light microscopy images reveal typical Bidder’s organ in close spatial relationship with ovary. In some cases, continuity between both organs is observed (Fig. 4).