Contributions to Zoology, 85 (4) – 2016Jonas Keiler; Stefan Richter; Christian S Wirkner: Revealing their innermost secrets: an evolutionary perspective on the disparity of the organ systems in anomuran crabs (Crustacea: Decapoda: Anomura)
Material and methods

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Resin injection

The acrylic casting resin Mercox 2-CL (Ladd Research, Williston, VT) or the polyurethane-based casting resin PU4ii (vasQtec, Zurich, Switzerland) was injected into the heart of defrosted specimens (K. pura­vida) or specimens previously killed using CO2 -saturated water (A. cholchol) or water mixed with 1 g / 100 mL clove powder (L. hirta). In a first step, PU4ii was mixed with an x-ray opaque additive (sublimated iodine or Tetric EvoFlow® composite (Ivoclar Vivadent, Eilwangen, Germany) dissolved in 2-butanone) while Mercox was used unadulterated (see S1). In a next step, the resin was mixed with the appropriate catalyst and placed in a 5 ml syringe (Luer Lock Solo, Braun, Melsungen, Germany) just before use. The resin in the syringe was injected via an injection cannula (diameter 0.3-0.6 mm, Sterican, Braun, Melsungen, Germany) through the carapace into the heart and the specimens were left for several minutes to allow the resin to polymerize and temper.