Contributions to Zoology, 69 (1/2) (2000)S. Bell; J.E. Bron; C. Sommerville: The distribution of exocrine glands in Lepeophtheirus salmonis and Caligus elongatus (Copepoda: Caligidae)

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Lepeophtheirus salmonis

DAB was successful in highlighting gland structures in all life-stages of L. salmonis. In chalimus, preadult and adult stages there was considerable variability between specimens of the same sample with regard to their staining characteristics. Pictures of gland distribution in these stages were therefore constructed from multiple specimens. The DAB stain also picked up some other, non-glandular features. In both L. salmonis and C. elongatus retinula cells of the eye and parts of the cerebrum often stained moderately with DAB, as did some of the body musculature, particularly the attachment points of the muscles to the cuticle. In specimens with damaged integuments it was common to see strong DAB-positive staining around the tears and cuts in the cuticle. It is not possible to say whether such staining is due to DAB-positive components in the exposed cuticle or whether this represents an active mechanism where DAB-positive products are aggregating at sites of injury.