Glochinema bathyperuvensis sp. n., the first record of Epsilonematidae from bathyal muddy sediments of the oxygen minimum zone (OMZ) off Callao, Peru (eastern Pacific Ocean) is described. The new species belongs to the subfamily Glochinematinae and is characterized by the possession of more than 8 pairs of subdorsal thorns asymmetrically arranged and a single dorsal one in the pharyngeal region, as well as by the presence of ten to thirteen blade-like cuticular protrusions forming a latero-dorsal palisade around the posterior head region and anterior cervical region. In addition, G. bathyperuvensis is characterized by its large size, more than 240 cuticular annules, provided with an extremely dense „hairy“ body ornamentation and, four rows of ambulatory setae: two inner subventral ones with 8 to 9 setae in males, and 5 to 6 setae in females, and two outer subventral rows composed each of 9–12 ambulatory setae followed by a large number of modified somatic setae. The dense hairy body spines, the large number of modified somatic setae, its dominant occurrence exclusive in oxygen-depleted, organic-rich soupy surface sediments indicate an adaptation to the stressed OMZ habitat conditions.